Whalen B J, Goldschneider I
Department of Pathology, University of Connecticut Health Center, Farmington 06030-3105.
Cell Immunol. 1993 Oct 1;151(1):168-86. doi: 10.1006/cimm.1993.1229.
Quantitative adoptive transfer assays were developed to detect the precursors of TI-1, TI-2, and TD antigen-reactive B cells in rat lymphoid tissues. Studies on the immune responses in normal and athymic nude rats validate the use of TNP-lipopolysaccharide as a TI-1 antigen, TNP-Ficoll as a TI-2 antigen, and SRBC as a TD antigen in rats. The precursors to these immunologically competent B cells are detected, following transfer into irradiated histocompatible recipients, by their ability to generate expanded populations of antigen-reactive B cells capable of mounting antibody responses (splenic IgM plaque-forming cells) to these antigens. Maximal numbers of antigen-reactive B cells emerge in antigenically naive rats after an interval of 7-12 days following transfer of donor lymphoid cells and decline rapidly thereafter. The delayed responses in adoptive recipients reconstituted with spleen cells are proportional to the numbers of spleen cells transferred and are shown to be primarily donor derived using histocompatible Ig kappa chain alloantigen disparate rat strain combinations. The precursors of TI-1, TI-2, and TD antigen-reactive B cells are present in both donor spleen and bone marrow. However, precursor cells to TI-1 and TD antigens are largely absent from donor lymph node cells, whereas precursors to the TI-2 antigen are as prevalent in donor lymph node as in donor spleen. These results support the hypothesis that newly formed virginal B cells represent transient populations of precursor cells that undergo further proliferation and differentiation in the spleen before acquiring immunological competence. The results also suggest that the precursors of TI-2 antigen-reactive B cells differ developmentally from those of TI-1 and TD antigen-reactive B cells, and that the antigen-reactive progeny of these precursors require additional stimulation in order to join the pool of long-lived peripheral B cells.
已开发出定量过继转移测定法，以检测大鼠淋巴组织中TI-1、TI-2和TD抗原反应性B细胞的前体细胞。对正常和无胸腺裸鼠免疫反应的研究证实，在大鼠中TNP-脂多糖可作为TI-1抗原，TNP-菲可作为TI-2抗原，绵羊红细胞可作为TD抗原。将这些具有免疫活性的B细胞的前体细胞转移至经辐照的组织相容性受体后，通过它们产生能够对这些抗原产生抗体反应（脾IgM斑块形成细胞）的抗原反应性B细胞扩增群体的能力来检测。在供体淋巴细胞转移后7-12天的间隔期后，抗原反应性B细胞的最大数量出现在无抗原经验的大鼠中，此后迅速下降。用脾细胞重建的过继受体中的延迟反应与转移的脾细胞数量成正比，并且使用组织相容性Igκ链同种抗原不同的大鼠品系组合显示主要来源于供体。TI-1、TI-2和TD抗原反应性B细胞的前体细胞存在于供体脾脏和骨髓中。然而，供体淋巴结细胞中基本不存在TI-1和TD抗原的前体细胞，而TI-2抗原的前体细胞在供体淋巴结中的含量与供体脾脏中的含量一样普遍。这些结果支持了这样的假设，即新形成的处女B细胞代表前体细胞的短暂群体，它们在获得免疫能力之前在脾脏中经历进一步的增殖和分化。结果还表明，TI-2抗原反应性B细胞的前体细胞在发育上与TI-1和TD抗原反应性B细胞的前体细胞不同，并且这些前体细胞的抗原反应性后代需要额外的刺激才能加入长寿外周B细胞库。
