Castelmur I, DiPaolo C, Bachmann M F, Hengartner H, Zinkernagel R M, Kündig T M
Institute for Experimental Immunology, Zürich, Switzerland.
Cell Immunol. 1993 Oct 15;151(2):460-6. doi: 10.1006/cimm.1993.1254.
Sensitivities of in vitro and in vivo assays for the detection of lymphocytic choriomeningitis virus (LCMV)-specific CTL were compared. Measurement of primary cytotoxicity was the least sensitive of all the tested assays. However, when the same 51Cr release assays were performed after in vitro restimulation, this in vitro method was found to be more sensitive than all of the five in vivo assays tested. Assessment of CTL-mediated protection against LCMV replication in spleens was most sensitive among the in vivo tests, followed by CTL-mediated resistance to intracerebral or intraperitoneal challenge infections with vaccinia-LCMV-recombinant virus. Inhibition of LCMV-induced choriomeningitis and of the footpad swelling reaction were least sensitive in detecting LCMV-specific CTL. As discussed, the presented sensitivity gradient may most probably be generalized.
比较了用于检测淋巴细胞性脉络丛脑膜炎病毒（LCMV）特异性CTL的体外和体内试验的敏感性。在所有测试的试验中，原发性细胞毒性的测量是最不敏感的。然而，当在体外再刺激后进行相同的51Cr释放试验时，发现这种体外方法比所测试的五种体内试验中的任何一种都更敏感。在体内试验中，评估CTL介导的对脾脏中LCMV复制的保护是最敏感的，其次是CTL介导的对痘苗-LCMV重组病毒脑内或腹腔内攻击感染的抵抗力。在检测LCMV特异性CTL方面，抑制LCMV诱导的脉络丛脑膜炎和足垫肿胀反应最不敏感。如前所述，所呈现的敏感性梯度很可能具有普遍性。
