Liu S Q
Union Hospital, Tongji Medical University, Wuhan.
Zhonghua Bing Li Xue Za Zhi. 1993 Apr;22(2):77-9.
The rapidly frozen bone marrow specimen was displaced in iced acetone at low temperature first and was embedded in low-temperature glycol methacrylate resin subsequently which was then ready for cryostat section preparation. Since there was no routine fixation required, so that the morphology features will be well preserved for further immunohistochemical and histochemical studies. By this technique, the enzyme activity and localization site will also be well preserved, particularly the lymphoid cell surface antigens, and it is also valuable for retrospective study of biopsy specimens.
快速冷冻的骨髓标本首先在低温下置于冰丙酮中置换，随后嵌入低温乙二醇甲基丙烯酸酯树脂中，然后即可用于冷冻切片制备。由于不需要常规固定，因此形态学特征将得到很好的保存，以便进行进一步的免疫组织化学和组织化学研究。通过这种技术，酶活性和定位位点也将得到很好的保存，特别是淋巴细胞表面抗原，这对于活检标本的回顾性研究也很有价值。
