Jiang C B, Yang H Z, Yang H P, Yang Y, Xu K J
Department of Parasitology, Shanghai Second Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(1):38-40.
The proteins and antigens of nine strains of Toxoplasma gondii were analyzed by SDS-PAGE and immunoblotting. These strains including RH strain, Zs-2 strain, CN strain and SH1, SH2, SH3, SH4, SH5, SH8 strains, were obtained from other laboratories or isolated from deformity fetus in our laboratory. SDS-PAGE analysis revealed that these strains were very close in the major bands of proteins and differences were observed only in part. There were common components in antigens of all strains using IgG antibody prepared from high-titre rabbit antisera raised against RH strain of Toxoplasma.
采用SDS-PAGE和免疫印迹法对9株弓形虫的蛋白质和抗原进行了分析。这些菌株包括RH株、Zs-2株、CN株以及SH1、SH2、SH3、SH4、SH5、SH8株，均来自其他实验室或由本实验室从畸形胎儿中分离得到。SDS-PAGE分析显示，这些菌株在蛋白质主要条带方面非常接近，仅在部分区域存在差异。使用针对弓形虫RH株制备的高滴度兔抗血清所产生的IgG抗体检测发现，所有菌株的抗原中均存在共同成分。
