Lobie P E, García-Aragón J, Lincoln D T, Barnard R, Wilcox J N, Waters M J
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Brain Res Dev Brain Res. 1993 Aug 20;74(2):225-33. doi: 10.1016/0165-3806(93)90008-x.
There is literature evidence that both growth hormone (GH) and its mediator, insulin-like growth factor 1 (IGF-1), are able to act upon neuronal and glial cells in the brain. We report here the location of the GH receptor in the brain of the rat and rabbit. Receptor distribution was determined by immunohistochemistry with GH receptor/binding protein (BP) specific monoclonal antibodies and by in situ hybridization with a [35S]riboprobe. GH receptor/BP immunoreactivity in the rat was most prominent in the neonate and declined with postnatal age. Receptor immunoreactivity was generalised with variation in immunoreactivity in regional areas. In the rat, strongest immunoreactivity was seen in layers 2, 3, 5 and especially layer 6 of the cerebral cortex, in neurones of the thalamus and hypothalamus, in Purkinje cells of the cerebellum, in neurones of the trapezoid body of the brainstem, and in retinal ganglion cells. Glial cells, notably astrocytes were also strongly reactive, along with ependyma of the choroid plexus, ventricular lining and pia mater. In the neonatal rabbit, strongest immunoreactivity was evident in layers 2 and 3 of the cerebral cortex, in pyramidal cells of the hippocampus, and in neurones of the inferior and superior colliculi, brain stem reticular formation, dorsal thalamus and hypothalamus. A similar distribution of GH receptor mRNA was seen by in situ hybridization. The ontogeny of GH receptor/BP mRNA in whole rat brain was quantified by solution hybridization-RNAse protection assay. Contrary to its ontogeny in the liver (Endocrinology, 113 (1983) 1325-1329) receptor mRNA decreased with postnatal age.(ABSTRACT TRUNCATED AT 250 WORDS)
有文献证据表明，生长激素（GH）及其介质胰岛素样生长因子1（IGF-1）均能够作用于大脑中的神经元和神经胶质细胞。我们在此报告大鼠和家兔大脑中生长激素受体的定位。通过使用生长激素受体/结合蛋白（BP）特异性单克隆抗体进行免疫组织化学以及使用[35S]核糖探针进行原位杂交来确定受体分布。大鼠中的生长激素受体/BP免疫反应性在新生儿中最为显著，并随着出生后的年龄增长而下降。受体免疫反应性呈全身性分布，区域内存在免疫反应性差异。在大鼠中，大脑皮层的第2、3、5层尤其是第6层、丘脑和下丘脑的神经元、小脑的浦肯野细胞、脑干梯形体的神经元以及视网膜神经节细胞中可见最强的免疫反应性。神经胶质细胞，尤其是星形胶质细胞以及脉络丛、脑室衬里和软脑膜的室管膜也有强烈反应。在新生家兔中，大脑皮层的第2和3层、海马体的锥体细胞以及下丘和上丘、脑干网状结构、背侧丘脑和下丘脑的神经元中可见最强的免疫反应性。通过原位杂交也观察到生长激素受体mRNA的类似分布。通过溶液杂交-RNA酶保护试验对整个大鼠大脑中生长激素受体/BP mRNA的个体发生进行了定量。与其在肝脏中的个体发生情况相反（《内分泌学》，113（1983）1325 - 1329），受体mRNA随着出生后的年龄增长而减少。（摘要截取自250字）
