Sugo T, Mas E, Abouakil N, Endo T, Escribano M J, Kobata A, Lombardo D
Institute of Medical Science, University of Tokyo, Japan.
Eur J Biochem. 1993 Sep 15;216(3):799-805. doi: 10.1111/j.1432-1033.1993.tb18200.x.
This study describes the structure of the N-linked oligosaccharide chains of bile-salt-dependent lipase isolated from the pancreatic juice of a normal donor. After hydrazinolysis, neutral sugar chains were separated from acidic chains by paper electrophoresis and were fractionated using serial column chromatography with immobilized lectins and Bio-Gel P-4 filtration. Structural analysis was performed by means of sequential glycosidase digestion and revealed that the neutral sugar chains are mainly of the biantennary complex type. Fucose residues were identified for some trimannosyl core structures and were alpha(1-6) or alpha(1-2) linked to the innermost GlcNAc residue and a terminal Gal residue, respectively. Sialyl residues were also involved in the oligosaccharide structures. Most of these structures have no linear N-acetyllactosamine repeats. Evidence from several approaches suggests that the sugar chains of the human pancreatic bile-salt-dependent lipase possess a blood-group-related antigenic determinant.
本研究描述了从正常供体胰液中分离出的胆汁盐依赖性脂肪酶的N-连接寡糖链的结构。肼解后，通过纸电泳将中性糖链与酸性链分离，并使用固定化凝集素和Bio-Gel P-4过滤的串联柱色谱法进行分级分离。通过顺序糖苷酶消化进行结构分析，结果表明中性糖链主要为双天线复合型。在一些三甘露糖核心结构中鉴定出岩藻糖残基，它们分别以α(1-6)或α(1-2)连接至最内层的GlcNAc残基和末端Gal残基。唾液酸残基也参与了寡糖结构。这些结构中的大多数没有线性N-乙酰乳糖胺重复序列。几种方法的证据表明，人胰胆汁盐依赖性脂肪酶的糖链具有血型相关的抗原决定簇。
