Hilbert D M, Holmes K L, Anderson A O, Rudikoff S
Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Eur J Immunol. 1993 Oct;23(10):2412-8. doi: 10.1002/eji.1830231006.
Significant immigration of peripheral T cells into SCID thymus was observed following reconstitution with normal Peyer's patch, mesenteric lymph node or peripheral lymph node cells. Immunohistologic and flow cytometric analyses reveal that T cells from these tissues are found in the thymus for as long as 177 days and can account for up to 67% of intrathymic cells. The returning cells express the CD3/T cell receptor alpha/beta complex, indicative of mature cells, and are equally divided among helper (CD4+CD8-) and cytotoxic (CD4-/CD8+) phenotypes. The immigration of peripheral T cells is not accompanied by the appearance of immature, double-positive (CD4+CD8+) thymocytes as seen in similar reconstitutions using bone marrow. Taken together, these results suggest that peripheral T cells from a variety of lymphoid organs may regularly re-enter the thymus and, thus, possibly play a role in normal thymic development.
在用正常派尔集合淋巴结、肠系膜淋巴结或外周淋巴结细胞重建后，观察到外周T细胞大量迁移至重症联合免疫缺陷（SCID）小鼠的胸腺。免疫组织学和流式细胞术分析显示，来自这些组织的T细胞在胸腺中可存在长达177天，并且可占胸腺内细胞的67%。返回的细胞表达CD3/T细胞受体α/β复合物，表明其为成熟细胞，并且在辅助性（CD4+CD8-）和细胞毒性（CD4-CD8+）表型之间平均分配。与使用骨髓进行的类似重建中所见情况不同，外周T细胞的迁移并未伴随着未成熟双阳性（CD4+CD8+）胸腺细胞的出现。综上所述，这些结果表明，来自各种淋巴器官的外周T细胞可能会定期重新进入胸腺，因此可能在正常胸腺发育中发挥作用。
