Cuff C F, Cebra C K, Rubin D H, Cebra J J
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Eur J Immunol. 1993 Jun;23(6):1333-9. doi: 10.1002/eji.1830230622.
Following intraduodenal priming of mice with reovirus, precursor cytotoxic T lymphocytes (pCTL) rapidly appear in intraepithelial lymphocytes (IEL) and Peyer's patches. These cells express CTL activity after secondary in vitro stimulation with reovirus-infected cells. Adoptive transfer of Peyer's patch lymphocytes from normal BALB/c mice into reovirus-infected CB.17 severe combined immunodeficiency mice results in the infection-dependent appearance of large numbers of both CD8+Thy-1+ and CD8-Thy-1+, IEL that express the alpha/beta T cell receptor (TcR). Phenotypic and functional characterization of IEL derived from conventionally reared, reovirus-infected mice also points to extensive similarities in the pCTL derived from Peyer's patches and IEL. As in the Peyer's patches, pCTL are persistent in the IEL compartment for up to 4 weeks after infection. A large percentage of IEL that are recovered from reovirus-primed mice after in vitro culture are CD8+Thy-1+ cells that express alpha/beta TcR. Furthermore, depletion experiments demonstrate that the CD8+Thy-1+ population mediates the virus-specific CTL activity. Using limiting dilution analyses, it was estimated that 7 days after intraduodenal infection the average frequency of virus-specific pCTL was 197/10(6) CD8+Thy-1+ IEL and 190/10(6) CD8+Thy-1+ Peyer's patch lymphocytes. Taken together, these observations provide evidence that specific cellular immunity to reovirus in IEL is mediated at least in part, by conventional cytotoxic T lymphocytes and that these cells are functionally and phenotypically similar to the pCTL derived from the Peyer's patches.
用呼肠孤病毒对小鼠进行十二指肠内启动后，前体细胞毒性T淋巴细胞（pCTL）迅速出现在上皮内淋巴细胞（IEL）和派尔集合淋巴结中。这些细胞在用呼肠孤病毒感染的细胞进行二次体外刺激后表达CTL活性。将正常BALB/c小鼠的派尔集合淋巴结淋巴细胞过继转移到呼肠孤病毒感染的CB.17严重联合免疫缺陷小鼠中，会导致大量表达α/βT细胞受体（TcR）的CD8⁺Thy-1⁺和CD8⁻Thy-1⁺ IEL依赖感染而出现。来自常规饲养、呼肠孤病毒感染小鼠的IEL的表型和功能特征也表明，来自派尔集合淋巴结和IEL的pCTL有广泛的相似性。与派尔集合淋巴结一样，感染后pCTL在IEL区持续存在长达4周。体外培养后从呼肠孤病毒启动的小鼠中回收的大部分IEL是表达α/βTcR的CD8⁺Thy-1⁺细胞。此外，耗竭实验表明，CD8⁺Thy-1⁺群体介导病毒特异性CTL活性。使用有限稀释分析估计，十二指肠内感染7天后，病毒特异性pCTL的平均频率为197/10⁶ CD8⁺Thy-1⁺ IEL和190/10⁶ CD8⁺Thy-1⁺派尔集合淋巴结淋巴细胞。综上所述，这些观察结果提供了证据，表明IEL中对呼肠孤病毒的特异性细胞免疫至少部分由常规细胞毒性T淋巴细胞介导，并且这些细胞在功能和表型上与来自派尔集合淋巴结的pCTL相似。
