Deussen A, Sonntag M, Vogel R
Zentrum für Physiologie, Heinrich-Heine-Universität Düsseldorf, Germany.
Exp Eye Res. 1993 Aug;57(2):129-34. doi: 10.1006/exer.1993.1107.
The effect of inhibition of the L-arginine-nitric oxide pathway on regional ocular blood flow was studied in anaesthetized Beagle dogs. Under control conditions (mean arterial blood pressure 105 +/- 6 mmHg) trapping of 11 and 15-microns-diameter tracer microspheres revealed the following regional blood flows (ml min-1 g-1): retina 0.065 +/- 0.016, choroid 5.72 +/- 0.32, ciliary body 0.77 +/- 0.11, iris 0.18 +/- 0.04. After i.v. infusion of nitro-L-arginine methylester (20 mg kg-1), a potent inhibitor of nitric oxide production from L-arginine, mean arterial blood pressure increased from 105 +/- 6 mmHg by 19% to a new steady-state level of 125 +/- 7 mmHg. This increase of arterial blood pressure extended over 3 hr and was reversible after i.v. infusion of L-arginine (100 mg kg-1 over 10 min). Despite the increase in blood pressure following infusion of nitro-L-arginine methylester blood flow of choroid, ciliary body, and iris were significantly decreased by 40, 40 and 48%, respectively. Retinal blood flow did not change significantly (-12%). These results suggest that the L-arginine-nitric oxide pathway is of major importance for the adjustment of uveal blood flow under resting physiological conditions.
在麻醉的比格犬中研究了抑制L-精氨酸-一氧化氮途径对眼部局部血流的影响。在对照条件下（平均动脉血压105±6 mmHg），捕获直径为11和15微米的示踪微球显示出以下局部血流（毫升·分钟⁻¹·克⁻¹）：视网膜0.065±0.016，脉络膜5.72±0.32，睫状体0.77±0.11，虹膜0.18±0.04。静脉注射硝基-L-精氨酸甲酯（20毫克·千克⁻¹），一种从L-精氨酸产生一氧化氮的强效抑制剂后，平均动脉血压从105±6 mmHg升高了19%，达到新的稳态水平125±7 mmHg。这种动脉血压升高持续3小时，在静脉注射L-精氨酸（10分钟内100毫克·千克⁻¹）后是可逆的。尽管注射硝基-L-精氨酸甲酯后血压升高，但脉络膜、睫状体和虹膜的血流分别显著降低了40%、40%和48%。视网膜血流没有显著变化（-12%）。这些结果表明，L-精氨酸-一氧化氮途径在静息生理条件下对葡萄膜血流的调节中起主要作用。
