Paz A, Ben-Baruch G, Marciano D, Egozi Y, Haklai R, Kloog Y
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
FEBS Lett. 1993 Oct 18;332(3):215-7. doi: 10.1016/0014-5793(93)80634-7.
High levels of prenylated protein methyltransferase are expressed in the developing rat cerebellum and are responsible for methylation of endogenous G-proteins and 50-52 kDa synaptosomal proteins. Enzyme activity in cerebellar synaptosomes of 3 week postnatal rats is 2-fold higher than that found in adult rat cerebellum. A 10-fold rise in activity occurs at the end of the second and during the third postnatal weeks, followed by a subsequent decline. Expression of the enzymes' substrates follows the same pattern. The high methyltransferase activity in 3-week-old cerebellum coincides with the period of granule cell migration and synaptogenesis, suggesting a regulatory role for the enzyme and its substrates in cerebellar ontogenesis.
高水平的异戊烯化蛋白甲基转移酶在发育中的大鼠小脑中表达，负责内源性G蛋白和50 - 52 kDa突触体蛋白的甲基化。出生后3周大鼠小脑突触体中的酶活性比成年大鼠小脑中的酶活性高2倍。在出生后第二周结束时和第三周期间，酶活性增加了10倍，随后下降。酶底物的表达遵循相同的模式。3周龄小脑中的高甲基转移酶活性与颗粒细胞迁移和突触形成期相吻合，表明该酶及其底物在小脑个体发生中起调节作用。
