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水母发光蛋白再生过程中二硫键的质谱证据。

Mass spectrometric evidence for a disulfide bond in aequorin regeneration.

作者信息

Ohmiya Y, Kurono S, Ohashi M, Fagan T F, Tsuji F I

机构信息

PRESTO, JRDC, Solar Energy Research Group, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.

出版信息

FEBS Lett. 1993 Oct 18;332(3):226-8. doi: 10.1016/0014-5793(93)80637-a.

DOI:10.1016/0014-5793(93)80637-a
PMID:8405461
Abstract

Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys145 and Cys152 and that the reduction of this bond is involved in the regeneration of aequorin.

摘要

用快速原子轰击质谱法分析了纯化的重组脱辅基水母发光蛋白在经二硫苏糖醇（DTT）还原前后的胰蛋白酶消化产物。结果表明，脱辅基水母发光蛋白在半胱氨酸145和半胱氨酸152之间含有一个二硫键，并且该键的还原参与了水母发光蛋白的再生过程。

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