镉离子对大脑丁酰胆碱酯酶的抑制动力学以及锌离子、钙离子或镁离子对被抑制酶的再激活作用。
Inhibition kinetics of brain butyrylcholinesterase by Cd2+ and Zn2+, Ca2+ or Mg2+ reactivates the inhibited enzyme.
作者信息
Cokuğraş A N, Tezcan E F
机构信息
Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
出版信息
Int J Biochem. 1993 Aug;25(8):1115-20. doi: 10.1016/0020-711x(93)90589-7.
PMID:8405652
Abstract
- The inhibition kinetics of sheep brain butyrylcholinesterase (BChE) (acylcholine acylhydrolase, EC 3.1.1.8) by Cd2+ and Zn2+ has been studied. 2. KS has been determined as 0.14 mM. Cd2+ and Zn2+ were the hyperbolic mixed-type inhibitors of BChE. Ca2+ and Mg2+ had no effect on the enzyme activity in the experimental conditions. 3. But when the enzyme was inhibited by 0.1 mM Cd2+ or Zn2+, Ca2+ and Mg2+ reactivated the inhibited form of BChE.
摘要
- 研究了Cd2+和Zn2+对绵羊脑丁酰胆碱酯酶（BChE，酰基胆碱酰基水解酶，EC 3.1.1.8）的抑制动力学。2. 测定KS为0.14 mM。Cd2+和Zn2+是BChE的双曲线混合型抑制剂。在实验条件下，Ca2+和Mg2+对酶活性无影响。3. 但是当酶被0.1 mM Cd2+或Zn2+抑制时，Ca2+和Mg2+使被抑制形式的BChE重新激活。
Zotero 插件