17β-雌二醇受体对鸡肝染色质中RNA聚合酶I和II活性的刺激作用。
Stimulation of RNA polymerase I and II activities by 17 beta -estradiol receptor on chick liver chromatin.
作者信息
Dierks-Ventling C, Bieri-Bonniot F
出版信息
Nucleic Acids Res. 1977 Feb;4(2):381-95. doi: 10.1093/nar/4.2.381.
The endogenous transcriptional capacity (RNA polymerase I and II activity) of liver chromatin from chicks treated with 17 beta-estradiol for 24 h (E 24) was double that of the controls. E 24 chromatin contained estradiol receptor activity while control chromatin did not. Its presence suggested an implication in the enhanced activities of RNA polymerases of E 24 chromatin. When semi-purified estradiol receptor was added to control chromatin, the endogenous transcriptional capacity of this chromatin was greatly increased. Studies with alpha-amanitin showed that both RNA polymerase I and II were stimulated by the estradiol receptor. This stimulation was observed as long as homology of the system was maintained. Solubilized homologous RNA polymerases were stimulated much less by the hormone complex in the presence of heterologous DNA than with homologous chromatin. Prokaryotic RNA polymerase could not be stimulated by chick liver estradiol receptor in the presence of heterologous DNA.
用17β-雌二醇处理24小时（E24）的雏鸡肝脏染色质的内源性转录能力（RNA聚合酶I和II活性）是对照组的两倍。E24染色质含有雌二醇受体活性，而对照染色质则没有。其存在表明与E24染色质RNA聚合酶活性增强有关。当将半纯化的雌二醇受体添加到对照染色质中时，该染色质的内源性转录能力大大增加。用α-鹅膏蕈碱进行的研究表明，RNA聚合酶I和II均受雌二醇受体刺激。只要系统保持同源性，就会观察到这种刺激。在存在异源DNA的情况下，与同源染色质相比，溶解的同源RNA聚合酶受激素复合物的刺激要少得多。在存在异源DNA的情况下，原核RNA聚合酶不能被鸡肝雌二醇受体刺激。
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