鸡肝染色质上卵黄蛋白原序列的体外转录
In vitro transcription of vitellogenin sequences on chick liver chromatin.
作者信息
Dierks-Ventling C
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3929-43. doi: 10.1093/nar/5.10.3929.
The in vitro transcription of chick liver chromatin before and after estrogen treatment was studied. Transcription was by endogenous as well as homologous exogenous RNA polymerase II and the products were analyzed for size and specific vitellogenin sequences. Quantitatively more RNA was synthesized from chromatin of 24 h estrogen-treated (E 24) chicks than from that of untreated chicks. In either case the size of transcribed RNA ranged from 5S to larger than 28S and most was between 5S and 18S. When a fraction enriched in estrogen receptor (E-Rec) complex was added to chromatin from untreated chicks, a dramatic shift of the RNA transcribed into heavier regions occurred. Analysis of RNA transcripts by hybridization to cDNAvit showed an equal number of sequences transcribed from E 24 chromatin and control; however, 13 times more specific sequences were transcribed in the presence of E-Rec complex. The results indicate the E-Rec complex exerts a regulatory function in the specific transcription of the vitellogenin gene.
研究了雌激素处理前后鸡肝脏染色质的体外转录情况。转录由内源性以及同源外源性RNA聚合酶II进行，并且对产物的大小和特异性卵黄生成素序列进行了分析。从经24小时雌激素处理（E24）的雏鸡染色质中合成的RNA在数量上比未经处理的雏鸡更多。在任何一种情况下，转录的RNA大小范围从5S到大于28S，大多数在5S和18S之间。当将富含雌激素受体（E-Rec）复合物的组分添加到未经处理的雏鸡的染色质中时，转录的RNA会发生显著的向较重区域的转移。通过与cDNAvit杂交对RNA转录本进行分析表明，从E24染色质和对照中转录的序列数量相等；然而，在E-Rec复合物存在的情况下，转录的特异性序列多出13倍。结果表明E-Rec复合物在卵黄生成素基因的特异性转录中发挥调节功能。
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