Takahashi E, Hitomi A, Nakamura Y
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Genomics. 1993 Jul;17(1):234-6. doi: 10.1006/geno.1993.1310.
We have constructed a high-resolution cytogenetic map of human chromosome 5 with 206 new cosmid clones by direct R-banding fluorescence in situ hybridization. The fluorescent signals of 206 clones were evenly distributed throughout chromosome 5, although they were sublocalized preferentially to R-positive bands. This high-resolution cytogenetic map with an average distance of nearly 1 Mb will serve as important resource for construction of a genetic linkage map, which is essential for positional cloning of responsible genes. Moreover, these mapping data provide many useful landmarks for the construction of contig maps of chromosome 5, as required in the Human Genome Project.
我们通过直接R显带荧光原位杂交技术，利用206个新的黏粒克隆构建了人类5号染色体的高分辨率细胞遗传学图谱。206个克隆的荧光信号均匀分布于整个5号染色体，尽管它们优先定位于R阳性带。这个平均间距近1兆碱基的高分辨率细胞遗传学图谱将成为构建遗传连锁图谱的重要资源，而遗传连锁图谱对于定位克隆相关基因至关重要。此外，这些定位数据为人类基因组计划所要求的构建5号染色体重叠群图谱提供了许多有用的界标。
