嗜铬粒蛋白A（Chga）基因座在小鼠12号染色体和大鼠6号染色体同源区域的定位。

Assignment of the chromogranin A (Chga) locus to homologous regions on mouse chromosome 12 and rat chromosome 6.

作者信息

Simon-Chazottes D, Wu H, Parmer R J, Rozansky D J, Szpirer J, Levan G, Kurtz T W, Szpirer C, Guenet J L, O'Connor D T

机构信息

Department of Medicine, University of California, San Diego 92161.

出版信息

Genomics. 1993 Jul;17(1):252-5. doi: 10.1006/geno.1993.1316.

DOI:10.1006/geno.1993.1316

PMID:8406464

Abstract

Chromogranin A is an acidic protein, stored and released with catecholamines, and is overexpressed in genetic hypertension. In the human genome, its locus has previously been positioned on the long arm of Chromosome 14 in the 14q32 region. As a first step toward evaluating its potential linkage with hereditary hypertension, we determined its chromosomal position in mouse and rat. Chromogranin A was present as a single-copy gene in both mouse and rat. Analysis of the allele distribution in an interspecific mouse backcross by single-strand conformation polymorphism positioned the chromogranin A locus on Chromosome 12, between Igh-C and D12Pas1. Evaluation of a rat/mouse somatic cell hybrid panel indicated that chromogranin A is on rat Chromosome 6. In each case (mouse, rat, and human), chromogranin A is in a conserved region with nearby markers including the immunoglobulin heavy chain locus.

摘要

嗜铬粒蛋白A是一种酸性蛋白，与儿茶酚胺一起储存和释放，在遗传性高血压中过表达。在人类基因组中，其基因座先前已定位在14号染色体长臂的14q32区域。作为评估其与遗传性高血压潜在连锁关系的第一步，我们确定了它在小鼠和大鼠中的染色体位置。嗜铬粒蛋白A在小鼠和大鼠中均以单拷贝基因形式存在。通过单链构象多态性分析种间小鼠回交中的等位基因分布，将嗜铬粒蛋白A基因座定位在12号染色体上，位于Igh-C和D12Pas1之间。对大鼠/小鼠体细胞杂交板的评估表明，嗜铬粒蛋白A位于大鼠6号染色体上。在每种情况下（小鼠、大鼠和人类），嗜铬粒蛋白A都与包括免疫球蛋白重链基因座在内的附近标记处于保守区域。