Oda T, Nishiyama K, Ichiyama A
Department of Biochemistyr, Hamamatsu University School of Medicine, Japan.
Genomics. 1993 Jul;17(1):59-65. doi: 10.1006/geno.1993.1283.
We have reported the isolation of genomic clones encoding serine:pyruvate aminotransferase (SPT; also named alanine:glyoxylate aminotransferase, AGT) (T. Oda, T. Funai, and A. Ichiyama, 1990, J. Biol. Chem. 265: 7513-7519). These clones contained the entire SPT/AGT gene of 10 kb. In this work, we characterized this gene. The SPT/AGT gene consists of 11 exons, and the exon-intron boundaries have typical splice donor and acceptor sequences. Determination of the nucleotide sequence up to -1.25 kb from the transcription initiation site revealed the presence of many putative cis elements, some of which may explain the transcriptional regulation of the SPT/AGT gene by glucagon and glucocorticoid. The nucleotide sequence around the 5' flanking region of the rat SPT/AGT gene and the whole gene organization were compared with those of the human SPT/AGT gene. No obvious similarities were observed in the 5' flanking region up to -1.25 kb from the initiation site of the gene, but exons 2 to 10 of the rat and human genes have identical sizes and show high similarities.
丙酮酸转氨酶（SPT；也称为丙氨酸：乙醛酸转氨酶，AGT）的基因组克隆的分离（T. 小田、T. 船井和A. 市山，1990年，《生物化学杂志》265: 7513 - 7519）。这些克隆包含10 kb的完整SPT/AGT基因。在这项工作中，我们对该基因进行了表征。SPT/AGT基因由11个外显子组成，外显子 - 内含子边界具有典型的剪接供体和受体序列。对转录起始位点上游至 -1.25 kb的核苷酸序列的测定揭示了许多推定的顺式元件的存在，其中一些可能解释了胰高血糖素和糖皮质激素对SPT/AGT基因的转录调控。将大鼠SPT/AGT基因5'侧翼区域周围的核苷酸序列和整个基因结构与人类SPT/AGT基因的进行了比较。在基因起始位点上游至 -1.25 kb的5'侧翼区域未观察到明显的相似性，但大鼠和人类基因的外显子2至10大小相同且显示出高度相似性。
