Gingrich J C, Shadravan F, Lowry S R
Human Genome Center, Lawrence Berkeley Laboratory, California 94720.
Genomics. 1993 Jul;17(1):98-105. doi: 10.1006/geno.1993.1289.
A fluorescence in situ hybridization (FISH) map of human chromosome 21 was compiled using yeast artificial chromosome (YAC) DNA probes that encode 28 markers physically and/or genetically mapped on the chromosome. Probes that recognize the centromere and rDNA repeat sequences in the p arm were also placed as reference markers on the FISH map. For each probe, the location of the fluorescence hybridization signal was measured on metaphase chromosomes with respect to fractional chromosome length (FL) from p-ter. The location of the markers was established with a standard error of +/- 1.9 Mb using from 9 to 63 FL measurements for each probe. The relative order and separation of the markers as determined by FISH are shown to correspond well to those of other maps of the chromosome. Fifty-one additional YAC and 86 cosmid clones were also localized by FISH with respect to the 30 markers on the chromosome. The cosmids, chosen at random from a flow-sorter chromosome 21 cosmid library, show some biases in chromosome distribution.
利用酵母人工染色体（YAC）DNA探针构建了人类21号染色体的荧光原位杂交（FISH）图谱，这些探针编码了28个在染色体上进行了物理和/或遗传定位的标记。识别着丝粒和p臂中rDNA重复序列的探针也被作为参考标记置于FISH图谱上。对于每个探针，在中期染色体上相对于从p端起的染色体长度分数（FL）测量荧光杂交信号的位置。通过对每个探针进行9至63次FL测量，确定标记的位置，标准误差为±1.9 Mb。FISH确定的标记的相对顺序和间隔与该染色体的其他图谱显示出良好的对应关系。另外51个YAC和86个黏粒克隆也通过FISH相对于染色体上的30个标记进行了定位。从流式分选的21号染色体黏粒文库中随机选择的黏粒在染色体分布上显示出一些偏向性。
