人体中甲苯的生物监测与DNA多态性

Biological monitoring of toluene and DNA polymorphism in humans.

作者信息

Kawamoto T, Matsuno K, Arashidani K, Yoshikawa M, Oyama T, Hattori Y, Kodama Y

机构信息

Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

Int Arch Occup Environ Health. 1993;65(1 Suppl):S131-3. doi: 10.1007/BF00381324.

DOI:10.1007/BF00381324

PMID:8406909

Abstract

The effects of genetic polymorphism in aldehyde dehydrogenase 2 (ALDH2) on alcohol intakes and toluene metabolism were studied in workers who handle toluene. The genotypes of ALDH2 were divided into three groups, which were the homozygous genotype of normal ALDH2 (NN), the homozygous genotype of an inactive one (DD) and the heterozygous genotype of normal and inactive ones (ND), using the polymerase chain reaction (PCR) method in a solution containing ddGTP. The alcohol intake of the DD group was very small. On the other hand, the alcohol intake of NN group was higher than that of DD group. That of ND group was about half way between those of NN group and DD group. The alteration of toluene metabolism by the polymorphism of ALDH2 was not clear because the exposure levels of the workers to toluene were low.

摘要

在接触甲苯的工人中，研究了乙醛脱氢酶2（ALDH2）基因多态性对酒精摄入量和甲苯代谢的影响。使用聚合酶链反应（PCR）方法，在含有ddGTP的溶液中，将ALDH2的基因型分为三组，即正常ALDH2的纯合基因型（NN）、无活性ALDH2的纯合基因型（DD）以及正常与无活性ALDH2的杂合基因型（ND）。DD组的酒精摄入量非常少。另一方面，NN组的酒精摄入量高于DD组。ND组的酒精摄入量约为NN组和DD组之间的一半。由于工人对甲苯的暴露水平较低，ALDH2基因多态性对甲苯代谢的改变并不明显。