Lisi A M, Kazlauskas R, Trout G J
Australian Government Analytical Laboratories, Pymble, N.S.W.
J Chromatogr. 1993 Aug 11;617(2):265-70. doi: 10.1016/0378-4347(93)80497-r.
A gas chromatographic-mass spectrometric procedure for the quantitation of urinary 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) has been developed in which the THC-COOH was derivatized to its corresponding methyl ester-methyl ether derivative by direct extractive alkylation using tetrahexylammonium (THA+) counter-ion and iodomethane dissolved in toluene. The procedure utilised a sample volume of 2 ml and gave a detection limit of 0.25 ng/ml. The inter-run and intra-run coefficients of variation were 7.0% and 4.8%, respectively. The inter-day standard curves were linear in the concentration range 0-300 ng/ml with a mean r = 0.9997 (n = 4).
已开发出一种气相色谱 - 质谱法用于定量测定尿中11 - 去甲 - Δ⁹ - 四氢大麻酚 - 9 - 羧酸（THC - COOH），其中通过使用溶解在甲苯中的四己基铵（THA⁺）抗衡离子和碘甲烷进行直接萃取烷基化，将THC - COOH衍生化为其相应的甲酯 - 甲醚衍生物。该方法使用2 ml的样品体积，检测限为0.25 ng/ml。批间和批内变异系数分别为7.0%和4.8%。日间标准曲线在0 - 300 ng/ml的浓度范围内呈线性，平均r = 0.9997（n = 4）。
