Mao Q M, Stockmann R, Prince I G, Hearn M T
Department of Biochemistry, Monash University, Clayton, Vic., Australia.
J Chromatogr. 1993 Aug 27;646(1):67-80. doi: 10.1016/s0021-9673(99)87008-3.
Analytical solutions for a mathematical model describing dynamic adsorption processes of proteins onto non-porous adsorbent particles in a finite bath are presented. The model, based on the Langmuir adsorption isotherm, has been applied to experimental data obtained with affinity and ion-exchange adsorbents. The external film mass transfer resistance, as well as the rate of surface interaction between proteins and adsorbents, have been taken into account. The model has been extended to the case of adsorption onto porous particles by employing a linear driving force approximation for describing mass transfer in the pore fluid. This approach enables the derivation of an effective overall liquid phase mass transfer coefficient, permitting subsequent adaptation of the analytical solutions developed for non-porous particles. The evaluation of the effective liquid phase mass transfer coefficients is also described. Examples of a comparison between predicted and experimental dynamic adsorption curves for both dye-affinity and ion-exchange systems are presented. The application of the model for predicting the optimum operating conditions is discussed.
本文给出了一个数学模型的解析解，该模型描述了蛋白质在有限浴中向无孔吸附剂颗粒上的动态吸附过程。基于朗缪尔吸附等温线的该模型已应用于通过亲和吸附剂和离子交换吸附剂获得的实验数据。已考虑了外膜传质阻力以及蛋白质与吸附剂之间的表面相互作用速率。通过采用线性驱动力近似来描述孔隙流体中的传质，该模型已扩展到吸附到多孔颗粒的情况。这种方法能够推导出有效的总液相传质系数，从而允许随后对为无孔颗粒开发的解析解进行调整。还描述了有效液相传质系数的评估。给出了染料亲和系统和离子交换系统预测动态吸附曲线与实验动态吸附曲线比较的示例。讨论了该模型在预测最佳操作条件方面的应用。
