石蜡包埋切线切片法对恶性雀斑样痣及恶性雀斑样痣黑色素瘤的处理：免疫过氧化物酶染色及补充垂直切片的效用
Management of lentigo maligna and lentigo maligna melanoma with paraffin-embedded tangential sections: utility of immunoperoxidase staining and supplemental vertical sections.
作者信息
Stonecipher M R, Leshin B, Patrick J, White W L
机构信息
Department of Dermatology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
出版信息
J Am Acad Dermatol. 1993 Oct;29(4):589-94. doi: 10.1016/0190-9622(93)70226-j.
BACKGROUND
The use of frozen sections in the management of lentigo maligna and lentigo maligna melanoma has been the focus of some controversy.
OBJECTIVE
Our purpose was to utilize paraffin-embedded tangential sections in the management of two cases of lentigo maligna and three cases of lentigo maligna melanoma.
METHODS
A modification of Mohs micrographic surgery using rush paraffin-embedded sections with adjunctive immunoperoxidase staining (HMB-45) and supplemental vertical sections was employed.
RESULTS
This method resulted in enhanced histologic evaluation of section margins and did not compromise the diagnosis of the primary invasive melanoma or Breslow measurements.
CONCLUSION
Mohs micrographic surgery modified by the use of rush paraffin-embedded sections allows adjunctive immunoperoxidase staining and supplemental vertical sections that may be helpful in the management of lentigo maligna and lentigo maligna melanoma.
背景
在恶性雀斑样痣和恶性雀斑样痣黑色素瘤的治疗中使用冰冻切片一直是一些争议的焦点。
目的
我们的目的是利用石蜡包埋的切线切片来治疗两例恶性雀斑样痣和三例恶性雀斑样痣黑色素瘤。
方法
采用一种改良的莫氏显微外科手术，使用快速石蜡包埋切片并辅以免疫过氧化物酶染色（HMB - 45）以及补充垂直切片。
结果
该方法增强了对切片边缘的组织学评估，且不影响原发性浸润性黑色素瘤的诊断或 Breslow 测量。
结论
通过使用快速石蜡包埋切片改良的莫氏显微外科手术允许进行辅助免疫过氧化物酶染色和补充垂直切片，这可能有助于恶性雀斑样痣和恶性雀斑样痣黑色素瘤的治疗。
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