Sriramarao P, Prakash O, Metcalfe D D, Rao P V
Department of Biochemistry, Indian Institute of Science, Bangalore.
J Allergy Clin Immunol. 1993 Oct;92(4):567-80. doi: 10.1016/0091-6749(93)90081-p.
Anti-idiotypic antibodies (Ab-2), which are the mirror images of idiotypic antibodies (Ab-1), may be useful as diagnostic reagents and for use as immunogen to induce antigen-specific immune responses.
To explore the biologic potential of Ab-2 as diagnostic reagents in allergic diseases, murine mouse (m) Ab-2 were raised by immunizing Balb/c mice with affinity purified rabbit (r) Ab-1 specific for the pollen of Parthenium hysterophorus, an allergenic weed that grows wild on the Indian subcontinent and in Australia, Mexico, and the southern United States. Affinity purified Parthenium-specific human (h)AB-1 could successfully inhibit the binding of mAb-2 to immobilized rAb-1. Further, Balb/c mice immunized with mAb-2 induced Parthenium-specific anti-anti-idiotypic IgE and IgG antibodies. Specificity of the Ab-2 was confirmed by the ability of Parthenium pollen extracts to inhibit the binding of allergen-specific IgE and IgG Ab-1 in the sera of patients with rhinitis to immobilized mAb-2. Parthenium-sensitive patients with rhinitis who had positive results on skin prick tests to Parthenium pollen extracts also responded with a positive skin reaction to mAb-2.
Our data demonstrate that Parthenium-specific mAb-2 may be of value as surrogate allergens in allergen standardization and for in vitro diagnosis.
抗独特型抗体（Ab-2）是独特型抗体（Ab-1）的镜像，可用作诊断试剂和免疫原以诱导抗原特异性免疫反应。
为探索Ab-2作为变应性疾病诊断试剂的生物学潜力，用对银胶菊花粉具有特异性的亲和纯化兔（r）Ab-1免疫Balb/c小鼠，制备鼠（m）Ab-2。银胶菊是一种在印度次大陆、澳大利亚、墨西哥和美国南部野生生长的致敏性杂草。亲和纯化的银胶菊特异性人（h）AB-1能够成功抑制mAb-2与固定化rAb-1的结合。此外，用mAb-2免疫的Balb/c小鼠诱导产生了银胶菊特异性抗抗独特型IgE和IgG抗体。银胶菊花粉提取物能够抑制鼻炎患者血清中变应原特异性IgE和IgG Ab-1与固定化mAb-2的结合，从而证实了Ab-2的特异性。对银胶菊花粉提取物皮肤点刺试验呈阳性的银胶菊敏感型鼻炎患者，对mAb-2皮肤反应也呈阳性。
我们的数据表明，银胶菊特异性mAb-2作为变应原标准化和体外诊断中的替代变应原可能具有价值。
