Sperber K, Sylvester C, Gollub E, Goswami S, Kalb T H, Druce H, Rutledge J, Marom Z
Department of Medicine, Mount Sinai Medical Center, New York, NY 10029.
J Allergy Clin Immunol. 1993 Oct;92(4):581-8. doi: 10.1016/0091-6749(93)90082-q.
We recently described a novel 68 kd mucus secretagogue (MMS-68) derived from human monocytes, pulmonary macrophages, and a macrophage hybridoma, clone 63. We detected MMS-68 in monocyte culture supernatants from patients with steroid-dependent asthma and in bronchoalveolar lavage fluid from patients with chronic bronchitis by antigen capture ELISA and in normal lung tissue by immunohistochemistry.
To determine a role for MMS-68 in the regulation of nasal mucus, we labeled human nasal turbinates with tritiated glucosamine and assayed for the ability of the previously purified MMS-68 (stock solution) to induce mucus-like glycoconjugate release (MLGC). We also performed immunohistochemistry stains with an anti-MMS-68 antibody (1-D-10) on frozen sections (n = 5) of nasal turbinates from patients with allergic and nonallergic rhinitis who were undergoing rhinoplasty and measured MMS-68 levels in nasal lavages from patients who were undergoing topical nasal histamine or methacholine challenge.
MMS-68 is a potent nasal MLGC secretagogue causing a dose-dependent increase in MLGC release in vitro. Staining revealed a subepithelial distribution for MMS-68. Antigen capture ELISA of nasal lavages demonstrated mean MMS-68 levels from saline control challenge of 0.9 +/- 0.5 micrograms MMS-68 per milligram of protein (n = 5), 8.6 +/- 1.4 micrograms MMS-68 per milligram of protein from histamine challenge and 20.7 +/- 2.3 micrograms MMS-68 per milligram of protein (n = 5) after methacholine challenge.
Taken together these data suggest that MMS-68 may play a role in the normal regulation of mucus secretion.
我们最近描述了一种新的68kd黏液促分泌素（MMS - 68），它源自人单核细胞、肺巨噬细胞以及巨噬细胞杂交瘤克隆63。我们通过抗原捕获酶联免疫吸附测定法（ELISA）在类固醇依赖型哮喘患者的单核细胞培养上清液中以及慢性支气管炎患者的支气管肺泡灌洗液中检测到了MMS - 68，并通过免疫组织化学方法在正常肺组织中检测到了它。
为了确定MMS - 68在调节鼻黏液中的作用，我们用氚标记的葡糖胺标记人鼻甲，并检测先前纯化的MMS - 68（储备溶液）诱导黏液样糖缀合物释放（MLGC）的能力。我们还用抗MMS - 68抗体（1 - D - 10）对接受鼻整形手术的变应性和非变应性鼻炎患者的鼻甲冰冻切片（n = 5）进行免疫组织化学染色，并测量接受局部鼻组胺或乙酰甲胆碱激发试验患者的鼻灌洗液中的MMS - 68水平。
MMS - 68是一种强效的鼻MLGC促分泌素，在体外可引起MLGC释放呈剂量依赖性增加。染色显示MMS - 68分布于上皮下。鼻灌洗液的抗原捕获ELISA显示，盐水对照激发试验的MMS - 68平均水平为每毫克蛋白质0.9±0.5微克MMS - 68（n = 5），组胺激发试验后为每毫克蛋白质8.6±1.4微克MMS - 68，乙酰甲胆碱激发试验后为每毫克蛋白质20.7±2.3微克MMS - 68（n = 5）。
综合这些数据表明，MMS - 68可能在黏液分泌的正常调节中起作用。
