Kadoya Y, Yamashina S
Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Japan.
J Histochem Cytochem. 1993 Nov;41(11):1707-14. doi: 10.1177/41.11.8409377.
We examined the development of mouse submandibular gland by light and electron microscopy and determined the distribution of the alpha 6 integrin subunit and laminin in this process by immunofluorescence and immunoelectron microscopy. At Days 13.5 and 14 of gestation alpha 6 was localized over the entire cell surface of undifferentiated epithelial cells of the terminal cluster. On Day 15 the expression of alpha 6 could no longer be detected over central cells in the proximal portion of branched terminal cluster, whereas peripheral cells were stained over the entire surface. On Day 17 of gestation to day of birth, alpha 6 expression was restricted to the basolateral surface of the differentiated acinotubular structure. Its expression on acinar cells was uniformly distributed throughout the basolateral membrane, but on duct cells stronger staining towards the basal surface was noted. Similar expression was observed in adult acinar and duct cells. Expression of the alpha 6-subunit at the cell-cell contact in an early stage and its expression at the basolateral surface in an advanced stage indicate that integrin containing alpha 6 plays a significant role in cell-cell and cell-substrate interaction. Stage and cell type-specific change of integrin expression may be significant for submandibular development.
我们通过光学显微镜和电子显微镜检查了小鼠下颌下腺的发育情况，并通过免疫荧光和免疫电子显微镜确定了在此过程中α6整合素亚基和层粘连蛋白的分布。在妊娠第13.5天和第14天，α6定位于终末簇未分化上皮细胞的整个细胞表面。在第15天，在分支终末簇近端的中央细胞上不再能检测到α6的表达，而周边细胞的整个表面都被染色。从妊娠第17天到出生当天，α6的表达局限于分化的腺泡管状结构的基底外侧表面。其在腺泡细胞上的表达均匀分布于整个基底外侧膜，但在导管细胞上，朝向基底表面的染色更强。在成年腺泡和导管细胞中也观察到类似的表达。α6亚基在早期细胞间接触处的表达及其在晚期基底外侧表面的表达表明，含α6的整合素在细胞间和细胞与基质的相互作用中起重要作用。整合素表达的阶段和细胞类型特异性变化可能对下颌下腺的发育具有重要意义。
