Sticherling M, Hetzel F, Schröder J M, Christophers E
Department of Dermatology, University of Kiel, Germany.
J Invest Dermatol. 1993 Oct;101(4):573-6. doi: 10.1111/1523-1747.ep12366023.
The neutrophil-activating peptide 1/interleukin 8 (NAP-1/IL-8) has in the past been extensively characterized biochemically as well as functionally. Effects of NAP-1/IL-8 on inflammatory cells like neutrophilic granulocytes and lymphocytes, as well as its production by several different cell types, point towards an important role in different inflammatory processes. Recently, monoclonal antibodies have helped to establish immunoassays for detecting the peptide. Using such antibodies, we have performed in vitro studies on the time- and stimulus-dependent production of IL-8 by endothelial cells as well as fibroblasts. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha) efficiently induced both focal intracellular expression as well as secretion of the peptide when tested by immunocytochemistry and enzyme-linked immunosorbent assay (ELISA). After stimulation with phorbol myristate acetate (PMA) and lipopolysaccharide (LPS), such effects were seen only in endothelial cells, whereas interferon (IFN)-gamma did not induce any pronounced effect on either of the cells tested. These studies demonstrated in vitro release of IL-8 by different cells upon specific stimulation, thus underlining the significance of the in vivo secretion of this peptide, as noted in recent studies.
中性粒细胞激活肽1/白细胞介素8（NAP-1/IL-8）过去已在生化和功能方面得到广泛表征。NAP-1/IL-8对嗜中性粒细胞和淋巴细胞等炎症细胞的作用，以及它由几种不同细胞类型产生，表明其在不同炎症过程中起重要作用。最近，单克隆抗体有助于建立检测该肽的免疫测定法。使用此类抗体，我们对内皮细胞和成纤维细胞产生IL-8的时间和刺激依赖性进行了体外研究。当通过免疫细胞化学和酶联免疫吸附测定（ELISA）检测时，肿瘤坏死因子-α（TNF-α）和白细胞介素-1α（IL-1α）有效诱导了该肽的局部细胞内表达以及分泌。在用佛波酯肉豆蔻酸酯（PMA）和脂多糖（LPS）刺激后，这种效应仅在内皮细胞中可见，而干扰素（IFN）-γ对所测试的任何一种细胞均未诱导出任何明显效应。这些研究证明了不同细胞在特定刺激下体外释放IL-8，从而强调了如最近研究所指出的该肽体内分泌的重要性。
