Mutasim D F, Vaughan A, Supapannachart N, Farooqui J
Department of Dermatology, University of Cincinnati College of Medicine, Ohio 45267-0523.
J Invest Dermatol. 1993 Oct;101(4):624-7. doi: 10.1111/1523-1747.ep12366084.
We investigated the presence of bullous pemphigoid antibodies in bullous pemphigoid sera that are negative by standard indirect immunofluorescence. We incubated each of four indirect immunofluorescence-positive bullous pemphigoid sera, seven indirect immunofluorescence-negative bullous pemphigoid sera, one indirect immunofluorescence-negative herpes gestationis serum, three indirect immunofluorescence-positive epidermolysis bullosa acquisita sera, five indirect immunofluorescence-negative epidermolysis bullosa acquisita sera, and two normal human sera with fresh human skin explants in medium 199 at 4 degrees C for 48 h. All bullous pemphigoid sera, herpes gestations serum, and the three indirect immunofluorescence-positive epidermolysis bullosa acquisita sera had IgG that bound the basement membrane zone of skin explants with moderate to marked intensity as demonstrated by immunofluorescence. Normal sera and indirect immunofluorescence-negative epidermolysis bullosa acquisita sera failed to bind the explant basement membrane zone. Immunoblotting of bullous pemphigoid sera showed five of seven indirect immunofluorescence-negative bullous pemphigoid sera to bind high-molecular weight and/or low-molecular weight bullous pemphigoid antigens from epidermal extracts. We conclude that the skin explant culture system is a very sensitive method for the detection of bullous pemphigoid antibodies in all bullous pemphigoid sera.
我们研究了标准间接免疫荧光检测为阴性的大疱性类天疱疮血清中是否存在大疱性类天疱疮抗体。我们将4份间接免疫荧光阳性的大疱性类天疱疮血清、7份间接免疫荧光阴性的大疱性类天疱疮血清、1份间接免疫荧光阴性的妊娠疱疹血清、3份间接免疫荧光阳性的获得性大疱性表皮松解症血清、5份间接免疫荧光阴性的获得性大疱性表皮松解症血清以及2份正常人血清，与新鲜的人皮肤外植体在199培养基中于4℃孵育48小时。所有大疱性类天疱疮血清、妊娠疱疹血清以及3份间接免疫荧光阳性的获得性大疱性表皮松解症血清均有IgG以中度至显著强度结合皮肤外植体的基底膜带，免疫荧光显示了这一点。正常血清和间接免疫荧光阴性的获得性大疱性表皮松解症血清未能结合外植体基底膜带。对大疱性类天疱疮血清进行免疫印迹分析显示，7份间接免疫荧光阴性的大疱性类天疱疮血清中有5份能结合来自表皮提取物的高分子量和/或低分子量大疱性类天疱疮抗原。我们得出结论，皮肤外植体培养系统是检测所有大疱性类天疱疮血清中大疱性类天疱疮抗体的一种非常敏感的方法。
