Zhang Z G, Chopp M, Chen H
Department of Neurology, Henry Ford Hospital, Detroit, MI 48202.
J Neurol Sci. 1993 Jul;117(1-2):240-4. doi: 10.1016/0022-510x(93)90179-3.
We investigated the effect of post-ischemic 30 degrees C hypothermia on transient middle cerebral artery (MCA) occlusion in the rat. Male Wistar rats (n = 27) were subjected MCA occlusion for 2 h by inserting a nylon filament into the internal carotid artery. Three groups of animals were studied: 1) normothermic ischemia and normothermic reperfusion (n = 13), 2) normothermic ischemia and 1 h of hypothermic reperfusion (n = 6), 3) normothermic ischemia and 3 h of hypothermic reperfusion (n = 6); monitoring of cerebral temperatures were performed on two additional rats. The animals were sacrificed after one week, and coronal sections were obtained and stained with hematoxylin and eosin (H/E) for histopathological examination and determination of infarct volume. The data indicate that both normothermic reperfusion and 1 h hypothermic reperfusion groups exhibited similar infarct volumes in the cortex and the basal ganglia, respectively (P > 0.1). The 3 h post-ischemic hypothermia group revealed a significant decrease in infarct volume in the cortex compared to the normothermic group (P < 0.05). However, the infarct volume of the basal ganglia was not significantly lessened by the 3 h post-ischemic hypothermia. Thus 3 h post-ischemic hypothermia provides preferential reduction of cell damage in the cortex, from 2 h of MCA occlusion in the rat.
我们研究了缺血后30摄氏度低温对大鼠大脑中动脉（MCA）短暂闭塞的影响。将雄性Wistar大鼠（n = 27）通过插入尼龙丝至颈内动脉进行大脑中动脉闭塞2小时。研究了三组动物：1）常温缺血和常温再灌注组（n = 13），2）常温缺血和1小时低温再灌注组（n = 6），3）常温缺血和3小时低温再灌注组（n = 6）；另外对两只大鼠进行脑温监测。一周后处死动物，获取冠状切片并用苏木精和伊红（H/E）染色进行组织病理学检查和梗死体积测定。数据表明，常温再灌注组和1小时低温再灌注组在皮质和基底神经节的梗死体积分别相似（P > 0.1）。与常温组相比，缺血后3小时低温组皮质梗死体积显著减小（P < 0.05）。然而，缺血后3小时低温并未使基底神经节的梗死体积显著减小。因此，缺血后3小时低温可优先减轻大鼠大脑中动脉闭塞2小时后皮质中的细胞损伤。
