Roorda J, Wood M B
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota 55905.
J Orthop Res. 1993 Sep;11(5):648-54. doi: 10.1002/jor.1100110506.
The limits of room temperature (24 degrees C) and hypothermic (4 degrees C) storage for preservation of endothelial eccrine function were investigated with use of an ex vivo perfusion apparatus model of the cannulated canine tibia. Norepinephrine-induced contraction of smooth muscles in both the presence and absence of acetylcholine was compared, with and without selective blockade of endothelial-derived relaxing factor (EDRF). At room temperature, release of EDRF deteriorated after 24 h and was absent after 48 h of storage. At 4 degrees C, endothelial eccrine activity appeared unimpaired for as long as 72 h of storage. From 96-192 h of storage at 4 degrees C, release of EDRF was suggested, but with an attenuation of its effect on smooth muscle. At 216 and 504 h of storage at 4 degrees C, no endothelial eccrine activity was demonstrated.
利用插管犬胫骨的体外灌注装置模型，研究了室温（24℃）和低温（4℃）保存内皮外分泌功能的限度。比较了在有和没有乙酰胆碱的情况下，去甲肾上腺素诱导的平滑肌收缩情况，以及在有和没有选择性阻断内皮衍生舒张因子（EDRF）的情况下。在室温下，储存24小时后EDRF的释放恶化，48小时后则不存在。在4℃时，内皮外分泌活性在长达72小时的储存期内似乎未受损害。在4℃储存96 - 192小时后，提示有EDRF的释放，但其对平滑肌的作用减弱。在4℃储存216和504小时后，未显示出内皮外分泌活性。
