在大肠杆菌中表达的嗜热脂肪芽孢杆菌法呢基二磷酸合酶的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of Bacillus stearothermophilus farnesyl diphosphate synthase expressed in Escherichia coli.

作者信息

Nakane H, Koyama T, Obata S, Osabe M, Takeshita A, Nishino T, Ogura K, Miki K

机构信息

Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

J Mol Biol. 1993 Oct 20;233(4):787-8. doi: 10.1006/jmbi.1993.1554.

DOI:10.1006/jmbi.1993.1554

PMID:8411181

Abstract

Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 A resolution. The diffraction pattern indicated that the space group is I4(1)22 with unit-cell dimensions of a = b = 114 A and c = 247 A. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase.

摘要

嗜热脂肪芽孢杆菌的耐热法呢基二磷酸合酶（EC 2.5.1.10）在大肠杆菌中过表达，已通过气相扩散法结晶。使用硫酸铵作为沉淀剂获得了四方晶体。这些晶体的X射线衍射分辨率约为3埃。衍射图谱表明空间群为I4(1)22，晶胞参数为a = b = 114埃，c = 247埃。据认为，不对称单元包含两到三个法呢基二磷酸合酶分子。