Frydman H M, Cadavid E O, Yokosawa J, Henrique Silva F, Navarro-Cattapan L D, Santelli R V, Jacobs-Lorena M, Graessmann M, Graessmann A, Stocker A J
Departamento de Bioquímica, Universidade de S. Paulo, Brazil.
J Mol Biol. 1993 Oct 20;233(4):799-803. doi: 10.1006/jmbi.1993.1556.
We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promotor region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.
我们已经绘制了已知存在于美洲髯毛蚊C-8 DNA胀泡中的唯一转录单元，并描述了一个cDNA克隆pRa C-8-22的分离和序列，该克隆包含从这个DNA胀泡转录的mRNA的几乎完整拷贝以及基因组克隆BSC8-0.9的部分序列，基因组克隆BSC8-0.9包含启动子区域和转录单元的其余部分。从cDNA中存在的开放阅读框预测的蛋白质特征表明它是独特的且是分泌型的。
