Soeda E
Nihon Rinsho. 1993 Sep;51(9):2246-51.
A method for construction of YAC (Yeast Artificial Chromosome) libraries with large inserts has been developed and promoted the ongoing project of human genome. Isolation by PCR screening charges the YAC clone with a unique tag of a pair of PCR primers at the defined chromosome site (Sequence Tagged Sites; STS). Current evaluation of YAC has revealed that larger YAC has more problems where rearrangements including deletion and chimera occur extensively in DNA molecules, presenting a limited use of this technology in mapping; the contig map with mega YACs will be substituted by some other system such as cosmids with which the human healthy and disease genes will be characterized.
一种构建大片段插入的酵母人工染色体（YAC）文库的方法已经开发出来，并推动了人类基因组计划的进行。通过PCR筛选进行分离，在特定染色体位点（序列标签位点；STS）用一对PCR引物的独特标签对YAC克隆进行标记。目前对YAC的评估表明，较大的YAC存在更多问题，包括DNA分子中广泛发生的缺失和嵌合体等重排，这使得该技术在图谱绘制中的应用有限；含有超大YAC的重叠群图谱将被其他系统取代，如黏粒系统，人类健康和疾病基因将通过黏粒系统进行鉴定。
