Tsukamoto M, Tada H
Second Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan.
Nihon Jinzo Gakkai Shi. 1993 Jul;35(7):791-5.
The effect of thrombospondin (TSP) on attachment, spreading, cell toxicity and proliferation of cultured rat mesangial cells (M cell) were investigated. The results are as follows: 1. Attachment and spreading of M cell on the culture plates coated with TSP were inhibited in a dose dependent manner, 2. 50 micrograms/ml TSP in the medium brought about the significant detachment of the adhered M cell. 3. TSP had no demonstrable effects on 51Cr-release from M cell, 4. 3H-thymidine uptake into M cell was inhibited by TSP in a dose dependent manner. These results indicate that TSP modulates attachment, spreading and proliferation of M cell. Therefore, it is suggested that overproduction of TSP in M cell under some pathophysiologic conditions such as diabetic nephropathy may influence the M cell functions in a self-suppression.
研究了血小板反应蛋白（TSP）对培养的大鼠系膜细胞（M细胞）的黏附、铺展、细胞毒性和增殖的影响。结果如下：1. M细胞在涂有TSP的培养板上的黏附和铺展受到剂量依赖性抑制；2. 培养基中50微克/毫升的TSP导致已黏附的M细胞显著脱离；3. TSP对M细胞释放51Cr没有明显影响；4. TSP以剂量依赖性方式抑制3H-胸腺嘧啶核苷掺入M细胞。这些结果表明TSP调节M细胞的黏附、铺展和增殖。因此，有人提出在某些病理生理条件下，如糖尿病肾病，M细胞中TSP的过度产生可能以自我抑制的方式影响M细胞的功能。
