Kondoh N
Department of Urology, Osaka Central Hospital, National Federation of Health Insurance Societies.
Nihon Hinyokika Gakkai Zasshi. 1993 Aug;84(8):1385-91. doi: 10.5980/jpnjurol1989.84.1385.
Recent reports have shown the existence of renin in Leydig cells of the rat testis and immunoreactive renin activity in the human testis. But we could not rule out the possibility that the renin found in the testis is contamination of plasma components by endocytosis. In the present study we tried to demonstrate the renin production in the human testis by the following experiments. At first, we measured the plasma renin activity and testosterone in the internal spermatic vein simultaneously after treatment with hCG and obtained the results that suggested hCG-induced renin secretion in the human testis. Next, we showed the hCG-dependent renin production from the cultured human Leydig cell tumors in vitro. Thirdly, we demonstrated the existence of renin mRNA in the human testisusing reverse transcriptase-polymerase chain reaction (RT-PCR). Fourthly, we detected significant signals of human renin mRNA in the human testicular tissues by in situ hybridization. From the above results, it was demonstrated that the human renin gene was hCG-dependently expressed in the human testis.
最近的报告显示，大鼠睾丸的间质细胞中存在肾素，人睾丸中存在免疫反应性肾素活性。但我们不能排除睾丸中发现的肾素是通过内吞作用污染血浆成分的可能性。在本研究中，我们试图通过以下实验来证明人睾丸中肾素的产生。首先，我们在注射人绒毛膜促性腺激素（hCG）后，同时测量了精索内静脉中的血浆肾素活性和睾酮，结果提示hCG可诱导人睾丸分泌肾素。其次，我们在体外证明了培养的人睾丸间质细胞瘤可产生依赖于hCG的肾素。第三，我们利用逆转录-聚合酶链反应（RT-PCR）证明了人睾丸中存在肾素mRNA。第四，我们通过原位杂交在人睾丸组织中检测到了人肾素mRNA的显著信号。从上述结果可以证明，人肾素基因在人睾丸中呈hCG依赖性表达。
