通过用神经氨酸酶处理细胞来检测抗红细胞抗体。

Detection of anti-red blood cell antibodies by treatment of the cells with neuraminidase.

作者信息

Katz A, Klajman A, Yaretzky A, Steiner Z P, Knyszynski A

出版信息

Scand J Haematol. 1977 Feb;18(2):98-104. doi: 10.1111/j.1600-0609.1977.tb02078.x.

DOI:10.1111/j.1600-0609.1977.tb02078.x

Abstract

Neuraminidase treated RBC were used to detect incomplete unbound anti RBC antibodies in the plasma of patients with immune diseases. 80% (48 out of 60) of the examined patients gave positive results with this method while all the 50 normal controls were negative. The indirect antiglobulin test was negative in all the patients. In the direct antiglobulin test only two RBC samples, whether treated with neuraminidase or not, gave positive results. The present study indicates that the assay of plasma on neuraminidase treated RBC may serve as a sensitive method for the detection of the presence of anti RBC antibodies in various immunological diseases.

摘要

用神经氨酸酶处理过的红细胞来检测免疫疾病患者血浆中不完全未结合的抗红细胞抗体。80%（60例中的48例）受检患者用此方法检测结果为阳性，而所有50名正常对照均为阴性。所有患者的间接抗球蛋白试验均为阴性。在直接抗球蛋白试验中，只有两个红细胞样本（无论是否用神经氨酸酶处理）检测结果为阳性。本研究表明，用神经氨酸酶处理过的红细胞检测血浆，可作为检测各种免疫疾病中抗红细胞抗体存在的一种灵敏方法。