Pruzanski W, Jacobs H, Saito S, Donnelly E M, Lui L C
Immunology Diagnostic and Research Centre, Wellesley Hospital, University of Toronto, Ontario, Canada.
Am J Hematol. 1987 Oct;26(2):167-74. doi: 10.1002/ajh.2830260207.
One hundred and twenty sera with monoclonal immunoglobulin M's (IgM's) were tested for cryptic cold agglutinin activity against papain- and neuraminidase-treated adult and cord red blood cells (RBC). Fifteen sera (12.5%) agglutinated papain-treated RBC. Fourteen of these 15 sera also agglutinated neuraminidase-treated RBC. Ten additional sera agglutinated neuraminidase-treated but not papain-treated cells. Three patterns were observed among agglutinators of papain-treated RBC: either predominant agglutination of adult RBC, or of cord RBC or equal reaction, thus resembling I, i and non-Ii patterns of conventional cold agglutinins. We propose to call these patterns, tentatively, Icr (cr for cryptic), icr, and non-Iicr, respectively. Purified monoclonal IgM's retained the above patterns. Eluates from enzyme-treated cells contained monoclonal IgM's. Investigation of neuraminidase-treated RBC has shown that in distinction to T-anti-T system (Thomsen-Friedenreich receptor system), the majority of sera with monoclonal IgM's agglutinated more strongly adult RBC, and some agglutinated more strongly cord RBC. There was no loss of agglutinating activity after repeat freezing and thawing of the sera. Thus, it seems that monoclonal IgM's which react with neuraminidase-treated RBC detect antigenic determinants different from the T antigen. Cryptic CA activity of IgM's should be added to the list of antibody activities of these monoclonal immunoglobulins.
检测了120份含有单克隆免疫球蛋白M（IgM）的血清，以检测其对木瓜蛋白酶和神经氨酸酶处理的成人及脐带红细胞（RBC）的隐匿性冷凝集素活性。15份血清（12.5%）凝集了木瓜蛋白酶处理的RBC。这15份血清中的14份也凝集了神经氨酸酶处理的RBC。另外10份血清凝集了神经氨酸酶处理但未用木瓜蛋白酶处理的细胞。在木瓜蛋白酶处理的RBC凝集者中观察到三种模式：要么主要凝集成人RBC，要么凝集脐带RBC，要么反应相同，因此类似于传统冷凝集素的I、i和非Ii模式。我们建议暂时将这些模式分别称为Icr（cr代表隐匿性）、icr和非Iicr。纯化的单克隆IgM保留了上述模式。酶处理细胞的洗脱液中含有单克隆IgM。对神经氨酸酶处理的RBC的研究表明，与T-抗T系统（汤姆森-弗里德赖希受体系统）不同，大多数含有单克隆IgM的血清对成人RBC凝集更强，有些对脐带RBC凝集更强。血清反复冻融后凝集活性没有丧失。因此，似乎与神经氨酸酶处理的RBC反应的单克隆IgM检测到的抗原决定簇不同于T抗原。IgM的隐匿性CA活性应添加到这些单克隆免疫球蛋白的抗体活性列表中。
