二维DNA分型的平行基因组分析

Parallel genome analysis by two-dimensional DNA typing.

作者信息

Mullaart E, de Vos G J, te Meerman G J, Uitterlinden A G, Vijg J

机构信息

Ingeny B. V., Leiden, The Netherlands.

出版信息

Nature. 1993 Sep 30;365(6445):469-71. doi: 10.1038/365469a0.

DOI:10.1038/365469a0

PMID:8413593

Abstract

By two-dimensional (2-D) DNA typing a restriction enzyme digest of genomic DNA can be resolved on the basis of both size and base-pair sequence and subsequently analysed by repeat probe hybridization to reveal sequence variants at multiple genomic sites in parallel. The system has been partly automated and allows for large-scale comparative analysis of complex genomes in a cost-effective manner.

摘要

通过二维（2-D）DNA分型，基因组DNA的限制性酶切消化产物可以根据大小和碱基对序列进行解析，随后通过重复探针杂交进行分析，以并行揭示多个基因组位点的序列变异。该系统已部分自动化，并能够以经济高效的方式对复杂基因组进行大规模比较分析。