Sánchez-Olea R, Peña C, Morán J, Pasantes-Morales H
Institute of Cell Physiology, National University of Mexico, Mexico City.
Neurosci Lett. 1993 Jun 25;156(1-2):141-4. doi: 10.1016/0304-3940(93)90458-w.
Regulatory volume decrease (RVD) in astrocytes was inhibited by the Cl- exchanger blockers 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), dipyridamole and niflumic acid but not by the Cl- channel inhibitors diphenylamine-2-carboxylate (DPC) and anthracene-9-carboxylate (9-AC). The volume activated efflux of [3H]taurine and [3H]D-aspartate (as marker for glutamate) was similarly affected by these compounds. However, neither RVD nor osmolyte fluxes were significantly reduced by removal of external Cl-, suggesting that an anion exchanger activity is not required for the volume regulatory process. Alternatively, these results suggest that the anion exchanger molecule may function as an unidirectional Cl- channel possibly permeable also to amino acids.
星形胶质细胞中的调节性容积减小（RVD）受到氯离子交换体阻滞剂4,4'-二异硫氰基芪-2,2'-二磺酸（DIDS）、双嘧达莫和氟尼酸的抑制，但不受氯离子通道抑制剂二苯胺-2-羧酸盐（DPC）和蒽-9-羧酸盐（9-AC）的抑制。这些化合物对[3H]牛磺酸和[3H]D-天冬氨酸（作为谷氨酸的标志物）的容积激活外排也有类似影响。然而，去除细胞外氯离子后，RVD和渗透溶质通量均未显著降低，这表明容积调节过程不需要阴离子交换体活性。另外，这些结果表明阴离子交换体分子可能作为一种单向氯离子通道发挥作用，也可能对氨基酸具有通透性。
