Fattaey A R, Helin K, Dembski M S, Dyson N, Harlow E, Vuocolo G A, Hanobik M G, Haskell K M, Oliff A, Defeo-Jones D
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Oncogene. 1993 Nov;8(11):3149-56.
The retinoblastoma gene product, pRB, regulates cell proliferation by binding to and inhibiting the activity of key growth promoting proteins. Several cellular proteins have been shown to bind directly to pRB and the genes encoding a number of them have been isolated. The protein product of one of these genes is the transcription factor E2F. We have now isolated cDNA clones that contain the full-length coding sequence of two other proteins, RBP1 and RBP2, cloned originally by their interaction with pRB. The products of the RBP1 and RBP2 genes are ubiquitously expressed, large (200 kDa for RBP1 and 195 kDa for RBP2) nuclear phosphoproteins with structural motifs that suggest a role in transcriptional regulation. In addition we have been able to identify complexes of pRB and RBP1 in vivo that are dissociated in the presence of purified human papillomavirus E7 protein.
视网膜母细胞瘤基因产物pRB通过与关键生长促进蛋白结合并抑制其活性来调节细胞增殖。已证明几种细胞蛋白可直接与pRB结合，并且编码其中一些蛋白的基因已被分离出来。这些基因之一的蛋白产物是转录因子E2F。我们现已分离出cDNA克隆，其包含另外两种蛋白RBP1和RBP2的全长编码序列，这两种蛋白最初是通过它们与pRB的相互作用而克隆得到的。RBP1和RBP2基因的产物在全身广泛表达，是大型（RBP1为200 kDa，RBP2为195 kDa）核磷蛋白，其结构基序表明它们在转录调控中发挥作用。此外，我们已经能够在体内鉴定出pRB和RBP1的复合物，该复合物在纯化的人乳头瘤病毒E7蛋白存在下会解离。
