Badier M, Guillot C, Delpierre S, Vanuxem P
Laboratoire de Physiologie respiratoire, Faculté de Médecine, Marseille.
Presse Med. 1993 Sep 4;22(25):1169-74.
This study was performed to compare two methods of aerosols delivery and inhalation in a bronchial provocation test with carbachol : the stocked method (S) where the aerosol is previously stocked in a spirometer bell before inhalation and the dosimeter method (D) where the aerosol is directly inhaled after nebulization. Fourteen subjects (seven normal and seven asthmatic) underwent bronchial challenges with the two techniques. Bronchial sensitivity was calculated on SRaw-dose response curves to carbachol. SGaw-dose response curves were also constructed and the slopes of these curves were used to measure bronchial reactivity. Within subjects sensitivity values were lower with the D method than with the S method (40 +/- 5 micrograms (S E) versus 450 +/- 50 micrograms respectively in asthmatics, 340 +/- 30 micrograms versus 2350 +/- 130 micrograms respectively in healthy subjects). Reactivity values were higher with the dosimeter method (2,7 +/- 0,7 x 10(-5) versus 27 +/- 4 x 10(-5) in healthy and 10 +/- 1 x 10(-5) versus 106 +/- 16 x 10(-5) in asthmatics). The dosimeter method had greater efficacy, was less time consuming and appeared to be a useful method for carrying out standardised non-specific bronchoprovocation test.
储存法（S），即在吸入前将气雾剂预先储存在肺量计钟罩中；以及剂量计法（D），即雾化后直接吸入气雾剂。14名受试者（7名正常人及7名哮喘患者）接受了这两种技术的支气管激发试验。根据对卡巴胆碱的比气道阻力-剂量反应曲线计算支气管敏感性。还构建了比气传导率-剂量反应曲线，并使用这些曲线的斜率来测量支气管反应性。在受试者内部，剂量计法的敏感性值低于储存法（哮喘患者中分别为40±5微克（标准误）和450±50微克，健康受试者中分别为340±30微克和2350±130微克）。剂量计法的反应性值更高（健康受试者中为2.7±0.7×10⁻⁵和27±4×10⁻⁵，哮喘患者中为10±1×10⁻⁵和106±16×10⁻⁵）。剂量计法具有更高的效能，耗时更少，似乎是进行标准化非特异性支气管激发试验的一种有用方法。
