Navarro-García F, López-Revilla R, Tstusumi V
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del IPN, México DF, Mexico.
Parasitol Res. 1993;79(6):517-22. doi: 10.1007/BF00931595.
Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lysates of the E. histolytica HM1 virulent strain. Exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates for potential difference, short-circuit current, and transmural resistance and (b) mucosal damage ranging from vacuolation at the bases and shortening of epithelial cells to the loss of intercellular junctions, destruction of microvilli, and necrosis of interglandular epithelial zones. This acute model of intestinal amebiasis is sensitive, fast, and reliable.
在人类中，分析溶组织内阿米巴滋养体与大肠之间的初始相互作用是不可能的，而在实验动物中进行分析也非常困难。为了克服这一障碍，我们用溶组织内阿米巴HM1毒力株的滋养体裂解物处理安装在Ussing型小室中的全层兔结肠段的管腔侧。暴露于裂解物长达90分钟对结肠产生了剂量和时间依赖性影响，包括：（a）电位差、短路电流和跨膜电阻的衰减率增加；（b）黏膜损伤，范围从基底空泡化、上皮细胞缩短到细胞间连接丧失、微绒毛破坏以及腺间上皮区坏死。这种急性肠道阿米巴病模型灵敏、快速且可靠。
