Egert D, Wolf H, Maass H, Trams G
Steroids. 1977 Jan;29(1):35-46. doi: 10.1016/0039-128x(77)90107-6.
After injection of radioactively labelled cortisol, the distribution of the radioactivity in the subcellular fractions of the rat uterus (nuclei, mitochondria, microsomes and 105 000 X g supernatant) was studied. In all fractions, radioactivity was observed and maxima were found 10, 20 and 50 min. after injection of the labelled hormone. Radioactivity was measured in all subcellular fractions even 180 min. after application of labelled cortisol. Additionally, radiolabelled progesterone and unlabelled cortisol in the ratio 1:1 or 1:2 (moles:moles) were injected into the animals. Studying the uptake of labelled progesterone in the subcellular fractions of the uterine tissue, revealed that no competition of unlabelled cortisol could be observed 10, 20 and 50 min. after application of the hormone mixture, compared with the control experiments. The results of this study give evidence that the progesterone uptake into rat uterus is specific and cannot be influenced by unlabelled cortisol.
注射放射性标记的皮质醇后，研究了放射性在大鼠子宫亚细胞组分（细胞核、线粒体、微粒体和105000×g上清液）中的分布。在所有组分中均观察到放射性，且在注射标记激素后10、20和50分钟出现最大值。即使在应用标记的皮质醇180分钟后，仍对所有亚细胞组分中的放射性进行了测量。此外，还以1:1或1:2（摩尔比）的比例将放射性标记的孕酮和未标记的皮质醇注射到动物体内。研究子宫组织亚细胞组分中标记孕酮的摄取情况发现，与对照实验相比，在应用激素混合物后10、20和50分钟，未观察到未标记皮质醇的竞争作用。本研究结果表明，孕酮进入大鼠子宫的过程具有特异性，不受未标记皮质醇的影响。
