Saiduddin S, Zassenhaus H P
Steroids. 1977 Feb;29(2):197-213. doi: 10.1016/0039-128x(77)90039-3.
Estradiol binding components in the cytosol and nuclear fractions of the ovary from immature rats (22-28 days old) were characterized by in vitro methods. Several of the biochemical characteristics of the estradiol binding components in the ovarian tissue were compared with the estradiol receptor from the uterus. The results suggest that the ovarian estradiol binding components are similar to the specific high affinity estradiol receptors in the uterus. In the cytosol of intact rat ovary a significant fraction of the total binding sites was found to be occupied, presumably by the endogenous estrogen. Following hypophysectomy there was a significant increase in the available cytosol binding sites. Evidence for translocation of cytosol receptor-estrogen (RE) complex to the nucleus was obtained for the ovary. The sedimentation properties of the RE complex of the ovary and the uterus are similar. The ovarian cytosol RE complex sediments at 7-8S in glycerol gradients at low ionic strength and at 4S in sucrose gradients at high ionic strength. Following extraction with 0.4 M KCl the ovarain nuclear RE complex sediments at 5S in sucrose gradients which is identical to that of the uterine nuclear receptor.
采用体外方法对未成熟大鼠（22 - 28日龄）卵巢胞质溶胶和细胞核组分中的雌二醇结合成分进行了表征。将卵巢组织中雌二醇结合成分的若干生化特性与子宫中的雌二醇受体进行了比较。结果表明，卵巢中的雌二醇结合成分与子宫中特异性高亲和力的雌二醇受体相似。在完整大鼠卵巢的胞质溶胶中，发现相当一部分总结合位点被占据，推测是被内源性雌激素占据。垂体切除术后，可利用的胞质溶胶结合位点显著增加。已获得卵巢中胞质溶胶受体 - 雌激素（RE）复合物向细胞核转位的证据。卵巢和子宫的RE复合物的沉降特性相似。卵巢胞质溶胶RE复合物在低离子强度的甘油梯度中沉降系数为7 - 8S，在高离子强度的蔗糖梯度中沉降系数为4S。用0.4M KCl提取后，卵巢细胞核RE复合物在蔗糖梯度中沉降系数为5S，这与子宫细胞核受体相同。
