Kashima T, Tiu S N, Merrill J E, Vinters H V, Dawson G, Campagnoni A T
Mental Retardation Research Center, University of California Los Angeles, School of Medicine 90024.
Cancer Res. 1993 Jan 1;53(1):170-5.
Two putative human oligodendroglioma cell lines were examined for the expression of the oligodendrocyte-associated genes, 2',3'-cyclic nucleotide-3'-phosphodiesterase, myelin basic protein, myelin proteolipid proteins, and myelin-associated glycoprotein. The expression of these genes also was examined in control astrocytoma and neuroblastoma cell lines. In addition, the expression of the non-oligodendrocyte-specific genes, glial fibrillary acidic protein (GFAP), neuron-specific enolase and neurofilaments (NF) NF-L and NF-M also were examined. All the cell lines expressed 2',3'-cyclic nucleotide 3'-phosphodiesterase, neuron-specific enolase, and vimentin, and none expressed myelin-associated glycoprotein. The "oligodendrocyte-specific" myelin proteolipid protein mRNAs and the "neuron-specific" NF-L mRNA were expressed in the two astrocytoma cell lines, which also expressed GFAP. Expression of intermediate filament protein genes was more restricted. The astrocytoma, neuroblastoma, and oligodendroglioma cell lines expressed only GFAP, NF-M, and cytokeratin K7, respectively. These results: (a) provide molecular data confirming the classification of the two cell lines as oligodendrogliomal and suggest that their molecular profiles are indicative of immature oligodendrocytes; (b) demonstrate the expression of cytokeratins in oligodendrogliomal cell lines and suggest that apparent GFAP expression in oligodendrogliomas detected by immunocytochemical methods may be due to cross-reactivity with cytokeratins, with which they share common polypeptide sequence; and (c) indicate that astrocytoma cell lines can exhibit a "mixed" phenotype, expressing genes associated with fully differentiated oligodendrocytes and neurons.
对两种假定的人少突胶质细胞瘤细胞系进行检测，以观察少突胶质细胞相关基因2',3'-环核苷酸-3'-磷酸二酯酶、髓鞘碱性蛋白、髓鞘蛋白脂蛋白和髓鞘相关糖蛋白的表达情况。同时也检测了对照星形细胞瘤和神经母细胞瘤细胞系中这些基因的表达。此外，还检测了非少突胶质细胞特异性基因胶质纤维酸性蛋白（GFAP）、神经元特异性烯醇化酶和神经丝（NF）NF-L和NF-M的表达。所有细胞系均表达2',3'-环核苷酸3'-磷酸二酯酶、神经元特异性烯醇化酶和波形蛋白，无一表达髓鞘相关糖蛋白。“少突胶质细胞特异性”的髓鞘蛋白脂蛋白mRNA和“神经元特异性”的NF-L mRNA在两种星形细胞瘤细胞系中表达，这两种细胞系也表达GFAP。中间丝蛋白基因的表达更为局限。星形细胞瘤、神经母细胞瘤和少突胶质细胞瘤细胞系分别仅表达GFAP、NF-M和细胞角蛋白K7。这些结果：（a）提供了分子数据，证实了这两种细胞系为少突胶质细胞瘤，并表明它们的分子谱表明其为未成熟的少突胶质细胞；（b）证明了细胞角蛋白在少突胶质细胞瘤细胞系中的表达，并表明免疫细胞化学方法检测到的少突胶质细胞瘤中明显的GFAP表达可能是由于与细胞角蛋白的交叉反应，它们具有共同的多肽序列；（c）表明星形细胞瘤细胞系可表现出“混合”表型，表达与完全分化的少突胶质细胞和神经元相关的基因。
