Hénin Y, Mandelbrot L, Henrion R, Pradinaud R, Coulaud J P, Montagnier L
Institut Pasteur, Paris, France.
J Acquir Immune Defic Syndr (1988). 1993 Jan;6(1):72-5.
In order to estimate the prevalence of viral excretion in cervicovaginal secretions, we made a cross-sectional study of 55 HIV-infected women. The patient population was diverse, including pregnant and nonpregnant women in different disease stages from three centers. Virus replication was found in the cell-free supernatant from 12 of 55 cervicovaginal samples (21.8%) by coculture on the CD4-positive cell line CEM-C113. In addition, cell-associated virus was detected in five of a subgroup of 22 samples testing negatively on cell-free supernatant. The prevalence of HIV in the cell-free supernatant was not related to disease stage, zidovudine therapy, transmission group, or history of sexually transmitted diseases. Excretion of HIV was significantly higher in our population of pregnant women (eight of 21, 38%) compared with an unmatched group of nonpregnant women (four of 34, 11.8%; p = 0.04). These results provide evidence of cell-free virus shedding as well as the presence of cell-associated virus in the genital secretions of HIV-infected women.
为了评估宫颈阴道分泌物中病毒排泄的流行情况，我们对55名感染HIV的女性进行了一项横断面研究。患者群体多样，包括来自三个中心处于不同疾病阶段的孕妇和非孕妇。通过在CD4阳性细胞系CEM-C113上进行共培养，在55份宫颈阴道样本中的12份（21.8%）的无细胞上清液中发现了病毒复制。此外，在无细胞上清液检测呈阴性的22份样本亚组中的5份中检测到了细胞相关病毒。无细胞上清液中HIV的流行率与疾病阶段、齐多夫定治疗、传播组或性传播疾病史无关。与未匹配的非孕妇组（34名中的4名，11.8%；p = 0.04）相比，我们的孕妇群体中HIV排泄明显更高（21名中的8名，38%）。这些结果提供了无细胞病毒脱落以及HIV感染女性生殖器分泌物中存在细胞相关病毒的证据。
