Vaccarello L, Kanbour A, Kanbour-Shakir A, Whiteside T L
Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania.
Int J Gynecol Pathol. 1993 Jan;12(1):41-50. doi: 10.1097/00004347-199301000-00006.
Tumor-infiltrating lymphocytes (TIL) from human ovarian tumors of low malignant potential (LMP) were studied in situ by immunohistology and characterized functionally after isolation from tumors. In comparison to ovarian carcinomas, borderline (LMP) ovarian tumors contained significantly fewer infiltrating leukocytes. Phenotypically, TIL from LMP tumors contained significantly fewer activated (HLA-DR+ or CD25+) lymphocytes than did fresh TIL from ovarian carcinomas. Also, percentages of CD3-CD56+ natural killer cells were higher in LMP than in ovarian carcinomas. TIL obtained from LMP tumors proliferated well in vitro in the presence of interleukin 2 (IL2) and tumor necrosis factor alpha (TNF alpha) but did not show a selective enrichment in CD3+CD8+T lymphocytes generally observed with TIL from ovarian carcinomas. Antitumor cytotoxicity against a panel of tumor cell targets of cultured TIL from LMP tumor did not parallel that of effectors generated from autologous peripheral blood lymphocytes. However, no significant enrichment in reactivity against autologous tumor cells was observed in long-term cultures of TIL from LMP tumors. Thus, considerable differences were observed between phenotypic and functional characteristics of lymphoid cells infiltrating LMP tumors and invasive ovarian carcinomas.
通过免疫组织学方法对人低恶性潜能（LMP）卵巢肿瘤中的肿瘤浸润淋巴细胞（TIL）进行原位研究，并在从肿瘤中分离后对其功能特性进行表征。与卵巢癌相比，交界性（LMP）卵巢肿瘤中浸润的白细胞明显较少。从表型上看，LMP肿瘤的TIL中活化（HLA-DR+或CD25+）淋巴细胞的数量明显少于卵巢癌新鲜TIL中的数量。此外，LMP中CD3-CD56+自然杀伤细胞的百分比高于卵巢癌。从LMP肿瘤中获得的TIL在白细胞介素2（IL2）和肿瘤坏死因子α（TNFα）存在的情况下在体外增殖良好，但未显示出卵巢癌TIL中普遍观察到的CD3+CD8+T淋巴细胞的选择性富集。LMP肿瘤培养的TIL对一组肿瘤细胞靶标的抗肿瘤细胞毒性与自体外周血淋巴细胞产生的效应细胞的细胞毒性不平行。然而，在LMP肿瘤TIL的长期培养中未观察到对自体肿瘤细胞反应性的显著富集。因此，在浸润LMP肿瘤的淋巴细胞和侵袭性卵巢癌的表型和功能特征之间观察到了相当大的差异。
