Amiel M L, Moos J, Tesarik J, Testart J
Institute National de la Santé et de la Recherche Médicale (INSERM), Clamart, France.
Mol Reprod Dev. 1993 Jan;34(1):81-6. doi: 10.1002/mrd.1080340113.
The effects of an antiserum (anti-COC) against ovulated mouse cumulus-oocyte complexes (COC) on in vitro fertilization of mouse oocytes were studied. Preincubation of ovulated COC with various concentrations of anti-COC led to dose-dependent impairment of fertilization rates as well as to a decrease in the number of spermatozoa attached to the zona pellucida. Anti-COC was used to probe Western blots of cumulus proteins. These cumuli were obtained from 2 experimental groups of mice corresponding to 2 different maturational stages (preovulatory immature COC or preovulatory mature COC). Two antigens (70 and 80 kDa) present in cumulus intercellular matrix from mature COC were only found as traces in matrix from immature COC. In addition, the protein pattern of the cumulus intercellular matrix was different from that of cumulus cells, whatever the COC maturational stage. These results indicate the appearance of new proteins in the cumulus oophorus during preovulatory expansion and are consistent with the contraceptive action of anti-COC.
研究了一种针对排卵小鼠卵丘-卵母细胞复合体（COC）的抗血清（抗COC）对小鼠卵母细胞体外受精的影响。用不同浓度的抗COC对排卵后的COC进行预孵育，导致受精率呈剂量依赖性受损，同时附着于透明带的精子数量减少。抗COC用于检测卵丘蛋白的蛋白质印迹。这些卵丘取自对应于2个不同成熟阶段（排卵前未成熟COC或排卵前成熟COC）的2组实验小鼠。在成熟COC的卵丘细胞间基质中存在的两种抗原（70和80 kDa）在未成熟COC的基质中仅为痕量。此外，无论COC的成熟阶段如何，卵丘细胞间基质的蛋白质模式都与卵丘细胞不同。这些结果表明在排卵前扩张期间卵丘中出现了新的蛋白质，并且与抗COC的避孕作用一致。
