Herold C D, Andree K, Herold D A, Felder R A
Department of Pathology, University of Virginia Health Science Center, Charlottesville 22908.
Clin Chem. 1993 Jan;39(1):143-7.
The measurement of glycohemoglobin (GHb) by boronate affinity chromatography is useful in monitoring long-term glucose control in diabetic subjects. The inherent disadvantage of this method is the hands-on time required because the hemoglobin fractions are separated on individual disposable columns. To overcome this disadvantage, we have programmed a Hamilton Microlab 2200 automated pipetting cartesian robot to complete the procedure, from the aspiration of blood from the sample-collection tube to the transfer of the separated hemoglobin fractions to a microtiter plate for absorbance measurement. This automated robotic system can analyze 96 specimens, including patients' samples and control material, in approximately 3 h. The precision (CV) of the method ranged from 1.6% to 3.5% within-run and from 2.7% to 3.5% day-to-day. The results correlated with those obtained with the Accuflex semiautomated robot, which used the identical disposable column, and those obtained with a Primus high-performance liquid chromatograph, which used a regenerated microparticle column. Automation of the GHb procedure allowed improved throughput, reduced labor cost, improved precision, and offered greater laboratory safety.
通过硼酸亲和色谱法测定糖化血红蛋白（GHb）有助于监测糖尿病患者的长期血糖控制情况。该方法固有的缺点是所需的人工操作时间较长，因为血红蛋白组分是在单个一次性柱上进行分离的。为克服这一缺点，我们对一台Hamilton Microlab 2200自动移液笛卡尔机器人进行了编程，使其能够完成从样品采集管吸取血液到将分离出的血红蛋白组分转移至微孔板进行吸光度测量的整个过程。这个自动化机器人系统大约能在3小时内分析96个样本，包括患者样本和对照材料。该方法的批内精密度（CV）在1.6%至3.5%之间，日间精密度在2.7%至3.5%之间。其结果与使用相同一次性柱的Accuflex半自动机器人以及使用再生微粒柱的Primus高效液相色谱仪所获得的结果相关。糖化血红蛋白检测程序的自动化提高了通量，降低了劳动力成本，提高了精密度，并提供了更高的实验室安全性。
