人绒毛肠上皮细胞的镶嵌分化：A 型非分泌者中血型 A 抗原的斑片状表达。
Mosaic differentiation of human villus enterocytes: patchy expression of blood group A antigen in A nonsecretors.
作者信息
Maiuri L, Raia V, Fiocca R, Solcia E, Cornaggia M, Norèn O, Sjostrom H, Swallow D, Auricchio S, Dabelsteen E
机构信息
Department of Pediatrics, II Medical School, University of Naples, Italy.
出版信息
Gastroenterology. 1993 Jan;104(1):21-30. doi: 10.1016/0016-5085(93)90831-v.
BACKGROUND
The authors have shown that a mosaicism of brush border antigens may occur spontaneously on enterocytes of small intestine in human adult-type hypolactasia. The present paper gives another example of spontaneously occurring mosaicism as indicated by the patchy expression of blood group antigens on villus enterocytes.
METHODS
Thirty-five individuals were examined by immunomorphological techniques with antibodies against blood group antigens.
RESULTS
In 4 of 16 A blood group individuals, the blood group antigens were expressed only in some villus enterocytes. The individuals with this mosaic pattern were all shown to be nonsecretors. The A antigen in the positive enterocytes of these individuals was only present as the ALe(b) structure, whereas ALe(y) and ALe(d) were also present in the secretors. The patches of positive enterocytes were randomly distributed along the villus wall.
CONCLUSIONS
A nonsecretor individuals express the blood group antigens only in some villus enterocytes; this mosaicism does not arise from a heterogeneous population of stem cells within the crypts but rather reflects subtle differences in the pattern of differentiation between monoclonally derived epithelial cells on the villus.
背景
作者已表明，在成人型低乳糖血症患者的小肠肠上皮细胞上，刷状缘抗原的镶嵌现象可能自发出现。本文给出了另一个自发出现的镶嵌现象的例子，即绒毛肠上皮细胞上血型抗原的斑片状表达。
方法
采用针对血型抗原的抗体，通过免疫形态学技术对35名个体进行检查。
结果
在16名A型血个体中，有4名个体的血型抗原仅在部分绒毛肠上皮细胞中表达。具有这种镶嵌模式的个体均为非分泌型。这些个体阳性肠上皮细胞中的A抗原仅以ALe(b)结构存在，而分泌型个体中还存在ALe(y)和ALe(d)。阳性肠上皮细胞的斑块沿绒毛壁随机分布。
结论
非分泌型个体仅在部分绒毛肠上皮细胞中表达血型抗原；这种镶嵌现象并非源于隐窝内干细胞群体的异质性，而是反映了绒毛上单克隆来源的上皮细胞分化模式的细微差异。
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