Sakai Y, Zhong R, Garcia B, Wall W J
Department of Surgery, University Hospital, University of Western Ontario, London, Canada.
Transplantation. 1993 Jan;55(1):18-23. doi: 10.1097/00007890-199301000-00005.
To determine the tolerance of old livers to prolonged preservation, livers from aged rats (corresponding to humans in the sixth to seventh decades of life) were transplanted after specific periods of cold preservation. Male BN/BiRij rats received orthotopic, arterialized liver grafts from either young (5 months) or old (25 to 28 month) donor rats after liver storage for 12 (n = 6), 24 (n = 6) or 30 (n = 10) hours in University of Wisconsin solution. Outcome was assessed by survival, liver enzymes after transplantation, and histology of the grafts. There were no significant differences in survival rates between recipients of old and young grafts. All rats survived after 12-hr and 24-hr preservation except one recipient of an old graft preserved for 24 hr. After 30-hr preservation recipients of old and young livers had identical survival rates (60%). There was a strong correlation between the highest postoperative AST and ALT and the duration of preservation in all groups (P < 0.0001), but only in the 24-hr preservation experiments was the ALT significantly higher in recipients of old grafts than in recipients of young livers (P = 0.025). Age of the donor did not significantly affect the peak AST, but there was a correlation between donor age and the highest postoperative ALT (P = 0.007). Although intracellular vacuolization was a prominent histological finding in more than half of the old livers at the end of preservation, it was not associated with an increase in mortality. It is concluded that under the ideal conditions provided in the experiments, old rat livers tolerate long preservation periods with satisfactory graft survival compared with young livers.
为了确定老龄肝脏对延长保存的耐受性，在特定的冷保存期后，对老年大鼠（相当于人类六七十岁）的肝脏进行移植。雄性BN/BiRij大鼠在威斯康星大学溶液中保存肝脏12小时（n = 6）、24小时（n = 6）或30小时（n = 10）后，接受来自年轻（5个月）或老年（25至28个月）供体大鼠的原位动脉化肝移植。通过存活率、移植后肝酶水平和移植肝组织学来评估结果。老年和年轻移植肝受体的存活率没有显著差异。除了1只接受24小时保存的老年移植肝受体外，所有大鼠在12小时和24小时保存后均存活。30小时保存后，老年和年轻肝脏受体的存活率相同（60%）。所有组中，术后最高AST和ALT与保存时间之间存在强相关性（P < 0.0001），但仅在24小时保存实验中，老年移植肝受体的ALT显著高于年轻肝脏受体（P = 0.025）。供体年龄对AST峰值没有显著影响，但供体年龄与术后最高ALT之间存在相关性（P = 0.007）。尽管在保存结束时，超过一半的老年肝脏组织学检查发现细胞内空泡化很明显，但这与死亡率增加无关。结论是，在实验提供的理想条件下，与年轻肝脏相比，老年大鼠肝脏能耐受较长的保存期，移植肝存活率令人满意。
